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PRINCIPLES OF THREE-DIMENSIONAL IMAGING IN CONFOCAL MICROSCOPES
by Min Gu (Victoria University of Technology, Australia)
This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.
Contents:
- Introduction
- Three-Dimensional Fourier Optics of a Thin
Lens
- Confocal Bright-Field Microscopy with a Point Detector
- Confocal Bright-Field Microscopy with a Finite-Sized Detector
- Confocal Single-Photon Fluorescence Microscopy
- Confocal Two-Photon Fluorescence Microscopy
- Fibre-Optical Confocal Microscopy
- Confocal Microscopy under Ultrashort Pulse Illumination
- Confocal Microscopy with High-Aperture Objectives
- Significance of Three-Dimensional Transfer Functions
Readership: Students and researchers in applied physics, life science
& biology and optics.
| 352pp |
Pub. date: Jul 1996 |
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